bsm-54272R [Primary Antibody]
DCP1A (3F4) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: DCP1A

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 55802

Swiss Prot: Q9NPI6

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 63


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human Liver; Probed with DCP1A (3F4) Monoclonal Antibody (bsm-54272R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with DCP1A (3F4) Monoclonal Antibody (bsm-54272R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with DCP1A (3F4) Monoclonal Antibody (bsm-54272R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with DCP1A (3F4) Monoclonal Antibody (bsm-54272R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with DCP1A (3F4) Monoclonal Antibody (bsm-54272R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with DCP1A (3F4) Monoclonal Antibody (bsm-54272R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of LOVO cells with DCP1A (3F4) Monoclonal Antibody (bsm-54272R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).