bsm-54260R [Primary Antibody]
GORASP1 (3G1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GORASP1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 64689

Swiss Prot: Q9BQQ3

Source: Recombinant protein within human GRASP65 aa 300-440

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Plays an important role in assembly and membrane stacking of the Golgi cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes. Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 46


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of MCF-7 cells with GORASP1 (3G1) Monoclonal Antibody (bsm-54260R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with GORASP1 (3G1) Monoclonal Antibody (bsm-54260R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Uterine tissue incubated with GORASP1 (3G1) Monoclonal Antibody (bsm-54260R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


IF(ICC) staining with GORASP1 (3G1) Monoclonal Antibody (bsm-54260R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with GORASP1 (3G1) Monoclonal Antibody (bsm-54260R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with GORASP1 (3G1) Monoclonal Antibody (bsm-54260R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Lane 1: THP-1 Cells; Lane 2: SKBR3; Probed with GORASP1 (3G1) Monoclonal Antibody (bsm-54260R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.