bsm-54259R [Primary Antibody]
SMARCC1 (5G1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SMARCC1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6599

Swiss Prot: Q92922

Source: Recombinant protein within human SMARCC1 aa 700-900

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. May stimulate the ATPase activity of the catalytic subunit of the complex. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 123 kDa (predicted), 150 kDa (observed)


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Thymus; Lane 2: HL-60 Cells; Probed with SMARCC1 (5G1) Monoclonal Antibody (bsm-54259R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with SMARCC1 (5G1) Monoclonal Antibody (bsm-54259R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with SMARCC1 (5G1) Monoclonal Antibody (bsm-54259R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of K562 cells with SMARCC1 (5G1) Monoclonal Antibody (bsm-54259R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).