bsm-54253R [Primary Antibody]
DDX6 (15C2) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: DDX6

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 1656

Swiss Prot: P26196

Source: Recombinant protein within human DDX6 aa 100-300

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

RCK, also known as DDX6 and P54, is a member of the DEAD-box RNA helicase family of proteins, all of which share common protein motifs. Found in most tissues, RCK is an unwindase that exhibits ATP-dependent RNA un-winding activity, as well as the ability to decay RNA in the 5'-3' direction. In non-malignant cells, RCK is associated with all processes of normal RNA metabolism including splicing, export and translation initiation. Mutations in the gene encoding RCK can cause the protein to be overexpressed, changing its function to that of an oncogene that positively regulates the expression of genes involved in cell growth and proliferation. It is believed that, through its unwindase activity, the main function of RCK is to downregulate mRNA expression and maintain normal transcriptional levels within the cell.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 54


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human Colon; Lane 2: SH-SY5Y Cells; Lane 3: SiHa Cells; Lane 4: K562 Cells; Probed with DDX6 (15C2) Monoclonal Antibody (bsm-54253R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with DDX6 (15C2) Monoclonal Antibody (bsm-54253R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with DDX6 (15C2) Monoclonal Antibody (bsm-54253R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Small Intestine tissue incubated with DDX6 (15C2) Monoclonal Antibody (bsm-54253R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with DDX6 (15C2) Monoclonal Antibody (bsm-54253R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.