VALIDATION IMAGES
Lane 1: PC-3M Cells; Lane 2: U937 Cells; Lane 3: SH-SY5Y Cells; Probed with VPS35 (7C4) Monoclonal Antibody (bsm-54248R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with VPS35 (7C4) Monoclonal Antibody (bsm-54248R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with VPS35 (7C4) Monoclonal Antibody (bsm-54248R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with VPS35 (7C4) Monoclonal Antibody (bsm-54248R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with VPS35 (7C4) Monoclonal Antibody (bsm-54248R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of 293T cells with VPS35 (7C4) Monoclonal Antibody (bsm-54248R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).