bsm-54234R [Primary Antibody]
NR0B1 (8C2) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NR0B1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 190

Swiss Prot: P51843

Source: Recombinant protein within human NR0B1/ Dax1 aa 1-150

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 52


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: A549 Cells; Probed with NR0B1 (8C2) Monoclonal Antibody (bsm-54234R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with NR0B1 (8C2) Monoclonal Antibody (bsm-54234R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with NR0B1 (8C2) Monoclonal Antibody (bsm-54234R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with NR0B1 (8C2) Monoclonal Antibody (bsm-54234R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Flow cytometric analysis of A549 cells with NR0B1 (8C2) Monoclonal Antibody (bsm-54234R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).