VALIDATION IMAGES
Lane 1: Hela Cells; Lane 2: NIH-3T3 Cells; Lane 3: Rat Kidney; Lane 4: Mouse Testis; Probed with NCK1 (7C2) Monoclonal Antibody (bsm-54207R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with NCK1 (7C2) Monoclonal Antibody (bsm-54207R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with NCK1 (7C2) Monoclonal Antibody (bsm-54207R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with NCK1 (7C2) Monoclonal Antibody (bsm-54207R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with NCK1 (7C2) Monoclonal Antibody (bsm-54207R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with NCK1 (7C2) Monoclonal Antibody (bsm-54207R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with NCK1 (7C2) Monoclonal Antibody (bsm-54207R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with NCK1 (7C2) Monoclonal Antibody (bsm-54207R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of Jurkat cells with NCK1 (7C2) Monoclonal Antibody (bsm-54207R) 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).