bsm-54190R [Primary Antibody]
Citrate synthase Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Citrate synthase

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 1431

Swiss Prot: O75390

Source: Recombinant protein within human Citrate synthetase aa 1-200

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Citrate synthase is found in nearly all cells capable of oxidative metabolism. The enzyme citrate synthase exists in nearly all living cells and stands as a pace-making enzyme in the first step of the citric acid cycle (or Krebs cycle). Citrate synthase is localized within eukaryotic cells in the mitochondrial matrix, but is encoded by nuclear DNA rather than mitochondrial. It is synthesized using cytoplasmic ribosomes, then transported into the mitochondrial matrix. Citrate synthase is commonly used as a quantitative enzyme marker for the presence of intact mitochondria.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 52


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Rat Epididymis tissue incubated with Citrate synthase (3C3) Monoclonal Antibody (bsm-54190R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Citrate synthase (3C3) Monoclonal Antibody (bsm-54190R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Citrate synthase (3C3) Monoclonal Antibody (bsm-54190R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Muscle tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Mouse Spleen tissue lysates; Lane 5: Rat Heart tissue lysates; Lane 6: Rat Muscle tissue lysates; Lane 7: Rat Cerebrum tissue lysates; Lane 8: Rat Spleen tissue lysates; Lane 9: Human 293T cell lysates; Lane 10: Human HeLa cell lysates; Lane 11: Human HL-60 cell lysates; Lane 12: Human HUVEC cell lysates; Lane 13: Human HepG2 cell lysates probed with Citrate synthase Monoclonal Antibody, Unconjugated (bsm-54190R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Flow cytometric analysis of Hela cells with Citrate synthase (3C3) Monoclonal Antibody (bsm-54190R) 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).