VALIDATION IMAGES
Lane 1: K562 Cells; Lane 2: HepG2 Cells; Lane 3: Raji Cells; Probed with HDAC9 (2C9) Monoclonal Antibody (bsm-54186R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with HDAC9 (2C9) Monoclonal Antibody (bsm-54186R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with HDAC9 (2C9) Monoclonal Antibody (bsm-54186R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
4% Paraformaldehyde-fixed Raji (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HDAC9) monoclonal Antibody, unconjugated (bsm-54186R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with HDAC9 (2C9) Monoclonal Antibody (bsm-54186R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.