VALIDATION IMAGES
Lane 1: PC-12 Cells; Lane 2: A549 Cells; Probed with LOXL2 (7D7) Monoclonal Antibody (bsm-54163R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with LOXL2 (7D7) Monoclonal Antibody (bsm-54163R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with LOXL2 (7D7) Monoclonal Antibody (bsm-54163R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with LOXL2 (7D7) Monoclonal Antibody (bsm-54163R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with LOXL2 (7D7) Monoclonal Antibody (bsm-54163R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with LOXL2 (7D7) Monoclonal Antibody (bsm-54163R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with LOXL2 (7D7) Monoclonal Antibody (bsm-54163R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of A549 cells with LOXL2 (7D7) Monoclonal Antibody (bsm-54163R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).