bsm-54154R [Primary Antibody]
CBX3 (4B4) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CBX3

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 11335

Swiss Prot: Q13185

Source: Recombinant protein within human CBX3 aa 1-150

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 20


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Hela cells with CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: PC-12 Cells; Lane 2: MCF-7 Cells; Probed with CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.