bsm-54153R [Primary Antibody]
PDX1 (34A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PDX1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3651

Swiss Prot: P52945

Source: Synthetic peptide within C-terminal human PDX1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated PDX1 (34A1) Monoclonal Antibody (bsm-54153R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated PDX1 (34A1) Monoclonal Antibody (bsm-54152R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody (green). The nuclear counterstain is DAPI (blue).


Paraformaldehyde-fixed, paraffin embedded Human pancreatic cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PDX1 (34A1) Monoclonal Antibody, Unconjugated (bsm-54153R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Human ASPC-1 cell lysates; Lane 2: Human Panc-1 cell lysates probed with PDX1 (34A1) Monoclonal Antibody, Unconjugated (bsm-54153R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.