bsm-54137R [Primary Antibody]
Galectin 1 (9H8) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: LGALS1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3956

Swiss Prot: P09382

Source: Recombinant protein within human Galectin 1 aa 1-150

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 14


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

IF(ICC) staining with Galectin 1 (9H8) Monoclonal Antibody (bsm-54137R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Rat Stomach tissue incubated with Galectin 1 (9H8) Monoclonal Antibody (bsm-54137R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with Galectin 1 (9H8) Monoclonal Antibody (bsm-54137R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of NIH-3T3 cells with Galectin 1 (9H8) Monoclonal Antibody (bsm-54137R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: HL-60 Cells; Lane 2: Human Skeletal Muscle; Probed with Galectin 1 (9H8) Monoclonal Antibody (bsm-54137R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.