bsm-54135R [Primary Antibody]
ELAVL1 (7A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ELAVL1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 1994

Swiss Prot: Q15717

Source: Recombinant protein within human HuR/ ELAVL1 aa 1-150

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation (By similarity). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 36


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of Jurkat cells with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Paraformaldehyde-fixed and paraffin-embedded Mouse Heart tissue incubated with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Breast tissue incubated with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat Lung tissue incubated with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Rat Spleen; Lane 2: Rat Heart; Lane 3: PC-12 Cells; Lane 4: Mouse Heart; Lane 5: Hela Cells; Probed with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.