bsm-54127R [Primary Antibody]
TXNIP (8A11) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TXNIP

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 10628

Swiss Prot: Q9H3M7

Source: Synthetic peptide within human TXNIP aa 50-150

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1).

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 44


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: PC-12 Cells; Lane 2: Human Skeletal Muscle; Lane 3: NIH-3T3 Cells; Lane 4: Hela Cells; Probed with TXNIP (8A11) Monoclonal Antibody (bsm-54127R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with TXNIP (8A11) Monoclonal Antibody (bsm-54127R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with TXNIP (8A11) Monoclonal Antibody (bsm-54127R) at 1:100 in NIH-3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with TXNIP (8A11) Monoclonal Antibody (bsm-54127R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with TXNIP (8A11) Monoclonal Antibody (bsm-54127R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Stomach Cancer tissue incubated with TXNIP (8A11) Monoclonal Antibody (bsm-54127R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Kidney tissue incubated with TXNIP (8A11) Monoclonal Antibody (bsm-54127R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Stomach tissue incubated with TXNIP (8A11) Monoclonal Antibody (bsm-54127R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Hela cells with TXNIP (8A11) Monoclonal Antibody (bsm-54127R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: Human MCF-7 cell lysates; Lane 2: Human HeLa cell lysates probed with TXNIP Monoclonal Antibody, Unconjugated (bsm-54127R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.