bsm-54115R [Primary Antibody]
KPNA2 (2D10) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: KPNA2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3838

Swiss Prot: P52292

Source: Recombinant protein within human KPNA2 aa 1-200

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 58


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela Cells; Lane 2: 293T Cells; Lane 3: HepG2 Cells; Lane 4: NIH-3T3 Cells; Lane 5: PC-12 Cells; Probed with KPNA2 (2D10) Monoclonal Antibody (bsm-54115R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Flow cytometric analysis of 293T cells with KPNA2 (2D10) Monoclonal Antibody (bsm-54115R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).