bsm-54112R [Primary Antibody]
KMT5A (2A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: KMT5A

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 387893

Swiss Prot: Q9NQR1

Source: Synthetic peptide within human KMT5A 50-100

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 42


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Kidney; Lane 2: Mouse Liver; Lane 3: 293T Cells; Lane 4: 293 Cells; Probed with KMT5A (2A1) Monoclonal Antibody (bsm-54112R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Flow cytometric analysis of 293T cells with KMT5A (2A1) Monoclonal Antibody (bsm-54112R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).