bsm-54088R [Primary Antibody]
TUBA4A (1F1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TUBA4A

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7277

Swiss Prot: P68366

Source: Human alpha Tubulin 4A aa 1-200

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively, spliced transcript variants encoding different isoforms have been found for this gene.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: A431 Cells; Lane 2: Rat Brain; Lane 3: NIH-3T3 Cells; Lane 4: PC-12 Cells; Probed with TUBA4A (1F1) Monoclonal Antibody (bsm-54088R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with TUBA4A (1F1) Monoclonal Antibody (bsm-54088R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with TUBA4A (1F1) Monoclonal Antibody (bsm-54088R) at 1:100 in NIH-3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with TUBA4A (1F1) Monoclonal Antibody (bsm-54088R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with TUBA4A (1F1) Monoclonal Antibody (bsm-54088R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Thyroid tissue incubated with TUBA4A (1F1) Monoclonal Antibody (bsm-54088R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with TUBA4A (1F1) Monoclonal Antibody (bsm-54088R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with TUBA4A (1F1) Monoclonal Antibody (bsm-54088R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with TUBA4A (1F1) Monoclonal Antibody (bsm-54088R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Hela cells with TUBA4A (1F1) Monoclonal Antibody (bsm-54088R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).