DATASHEET
Host:
Rabbit
Target Protein:
Zic1
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
7545
Swiss Prot:
Q15915
Source:
Human Zic1 aa 1-100
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Background:
Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally-located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'.
VALIDATION IMAGES
Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated Zic1 (3G7) Monoclonal Antibody (bsm-54063R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated Zic1 (3G7) Monoclonal Antibody (bsm-54063R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of SH-SY5Y cells with Zic1 (3G7) Monoclonal Antibody (bsm-54060R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).