bsm-54040R
[Primary Antibody]
MMP9 Recombinant Antibody
DATASHEET
Host: Rabbit
Target Protein: MMP9
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 4318
Swiss Prot: P14780
Source: C terminal human MMP9
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store for 1 year at -20°C from date of shipment. Store for 1 year at -20°C from date of shipment.
Background:
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Size: 100ul
Concentration: Lot dependent
Predicted Molecular Weight: 100
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wenjun He. et al. Molecular Mechanism of Naringenin Against High-Glucose-Induced Vascular Smooth Muscle Cells Proliferation and Migration Based on Network Pharmacology and Transcriptomic Analyses.. FRONT PHARMACOL. 2022 Jun;13:862709-862709Read more>>
- Ding Lingli. et al. Ginsenoside compound-K attenuates OVX-induced osteoporosis via the suppression of RANKL-induced osteoclastogenesis and oxidative stress. NAT PRODUCT BIOPROSP. 2023 Dec;13(1):1-12Read more>>
VALIDATION IMAGES
IF(ICC) staining with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:100 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MMP9 (7D6) Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:200 overnight at 4°C, DAB staining.
Flow cytometric analysis of A431 cells with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MMP9 (7D6) Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Rat Spleen tissue lysates; Lane 2: Rat Lung tissue lysates probed with MMP9 (7D6) Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Normal human U-2 OS cell lysates; Lane 2: U-2 OS treated with 200nM TPA for 48 hours lysates; Lane 3: Normal human MDA-MB-231 cell lysates; Lane 4: MDA-MB-231 serum starved overnight, then treated with 200nM TPA for 24 hours probed with MMP9 Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with MMP9 monoclonal antibody, unconjugated (bsm-54040R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
U2OS (H) cells were treated with or without TPA (200nM,48h) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with MMP9 monoclonal antibody, unconjugated (bsm-54040R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
MDA-MB-231 (H) cells were treated with or without TPA (200nM,24h) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with MMP9 monoclonal antibody, unconjugated (bsm-54040R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Monoclonal Antibody, Unconjugated(bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Monoclonal Antibody, Unconjugated(bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Monoclonal Antibody, Unconjugated(bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Monoclonal Antibody, Unconjugated(bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Monoclonal Antibody, Unconjugated(bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.