bsm-54035R
[Primary Antibody]
GOLM1 (1F10) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: GOLM1
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 51280
Swiss Prot: Q8NBJ4
Source: Human GOLM1 aa 300-400
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
GP73 (also known as Golgi phosphoprotein 2, GOLPH 2 or Golgi membrane protein), is a widely expressed, epithelial-specific, type II transmembrane protein which resides in the Golgi apparatus, where it is responsible for the posttranslational modification of proteins produced in the Rough ER while assisting in the transport of proteins through the Golgi. The human GP73 gene has been mapped within a BAC and localized to chromosome 9q21.33. GP73 levels rise in those who have been diagnosed with acute and chronic liver diseases. Cellular response protein to viral infection.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: SH-SY5Y Cells; Probed with GOLM1 (1F10) Monoclonal Antibody (bsm-54035R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with GOLM1 (1F10) Monoclonal Antibody (bsm-54035R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with GOLM1 (1F10) Monoclonal Antibody (bsm-54035R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with GOLM1 (1F10) Monoclonal Antibody (bsm-54035R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse Small Intestine tissue incubated with GOLM1 (1F10) Monoclonal Antibody (bsm-54035R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with GOLM1 (1F10) Monoclonal Antibody (bsm-54035R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of Hela cells with GOLM1 (1F10) Monoclonal Antibody (bsm-54035R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).