bsm-54033R
[Primary Antibody]
Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: MYH11
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 4629
Swiss Prot: P35749
Source: C terminal human smooth muscle Myosin heavy chain 11
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter. The pericentric inversion of chromosome 16[inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation. Alternatively spliced transcript variants encoding different isoforms have been identified. MYH11 is a smooth muscle myosin belonging to the myosin heavy chain family. It is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP.
Size: 100ul
Concentration: Lot dependent
Cross Reactive Species:
Human
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
IF(ICC) staining with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Jejunum tissue incubated with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Trachea tissue incubated with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Stomach Cancer tissue incubated with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.