bsm-54027R [Primary Antibody]
TNFRSF10B (1D8) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TNFRSF10B

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 8795

Swiss Prot: O14763

Source: Human DR5 aa 200 to the C-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated by two distinct cell surface receptors, designated TNF-R1 and TNF-R2, which are expressed on most cell types. TNF function is primarily mediated through TNF-R1 signaling. Both receptors belong to the growing TNF receptor superfamily which includes FAS antigen and CD40. TNF-R1 contains a cytoplasmic motif, termed the "death domain," that has been found to be necessary for the transduction of the apoptotic signal. The death domain is also found in several other receptors, including FAS, DR2 (or TRUNDD), DR3 (Death Receptor 3), DR4 and DR5. TRUNDD, DR4 and DR5 are receptors for the apoptosis-inducing cytokine TRAIL. A non-death domain-containing receptor, designated decoy receptor (DcR1 or TRID), also specifically associates with TRAIL and may play a role in cellular resistance to apoptotic stimuli.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
(WB(1:500), IHC-P(1:50-200), IF(IHC-P)(1:50-200), FCM(1:50-100) )

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: HL-60 Cells; Probed with TNFRSF10B (1D8) Monoclonal Antibody (bsm-54027R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with TNFRSF10B (1D8) Monoclonal Antibody (bsm-54027R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with TNFRSF10B (1D8) Monoclonal Antibody (bsm-54027R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Carcinoma tissue incubated with TNFRSF10B (1D8) Monoclonal Antibody (bsm-54027R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.


Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with TNFRSF10B (1D8) Monoclonal Antibody (bsm-54027R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of SW480 cells with TNFRSF10B (1D8) Monoclonal Antibody (bsm-54027R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).