bsm-54019R
[Primary Antibody]
ABCC2 (2G9) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: ABCC2
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 1244
Swiss Prot: Q92887
Source: Human ABCC2 aa 800-1000
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Multi-drug resistance protein 2 (MRP2), also known as ABCC2, is an ATP binding cassette (ABC) transporter responsible for biliary excretion of xenobiotics, endobiotics, and their metabolites. Deficiency in ABCC2 results in the clinical disorder Dubin-Johnson syndrome. MRP2 is found to be expressed in a variety of human cancers, and is associated with resistance of tumor cells to various anticancer drugs including cisplatin. The predicted molecular weight of MRP2 is 174 kDa, while mature MRP2 usually has a slower migration around 190-250 kDa due to the glycosylation. Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
IF(ICC) staining with ABCC2 (2G9) Monoclonal Antibody (bsm-54019R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with ABCC2 (2G9) Monoclonal Antibody (bsm-54019R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with ABCC2 (2G9) Monoclonal Antibody (bsm-54019R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counter stained with hematoxylin
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with ABCC2 (2G9) Monoclonal Antibody (bsm-54019R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with ABCC2 (2G9) Monoclonal Antibody (bsm-54019R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of A549 cells with ABCC2 (2G9) Monoclonal Antibody (bsm-54019R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).