bsm-54014R
[Primary Antibody]
Profilin 1 (4A11) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Profilin 1
Clonality: Monoclonal
Isotype: IgG
Swiss Prot: P07737
Source: Full length protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 15 kDa
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Hela cell probed with Profilin 1 (4A11) Monoclonal Antibody (bsm-54014R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
IF(ICC) staining with Profilin 1 (4A11) Monoclonal Antibody (bsm-54014R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Profilin 1 (4A11) Monoclonal Antibody (bsm-54014R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Profilin 1 (4A11) Monoclonal Antibody (bsm-54014R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded mouse colon tissue incubated with Profilin 1 (4A11) Monoclonal Antibody (bsm-54014R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded mouse kidney tissue incubated with Profilin 1 (4A11) Monoclonal Antibody (bsm-54014R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Profilin 1 (4A11) Monoclonal Antibody (bsm-54014R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of Hela cells with Profilin 1 (4A11) Monoclonal Antibody (bsm-54014R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).