bsm-52967R
[Primary Antibody]
Bid (9C9) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Bid
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 637
Swiss Prot: P55957
Source: Full length protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Human thymus cells lysates probed with Bid (9C9) Monoclonal Antibody (bsm-52967R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Flow cytometric analysis of Hela cells with Bid (9C9) Monoclonal Antibody (bsm-52967R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Bid (9C9) Monoclonal Antibody (bsm-52967R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with Bid (9C9) Monoclonal Antibody (bsm-52967R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with Bid (9C9) Monoclonal Antibody (bsm-52967R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with Bid (9C9) Monoclonal Antibody (bsm-52967R) at 1:100 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Bid (9C9) Monoclonal Antibody (bsm-52967R) at 1:100 in PC-3M cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Bid (9C9) Monoclonal Antibody (bsm-52967R) at 1:100 in SW480 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.