bsm-52966R
[Primary Antibody]
MyoD1 (4C4) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: MyoD1
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 4654
Swiss Prot: P15172
Source: Human MyoD1 between 200-600 amino acids
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Hela cells lysates probed with MyoD1 (4C4) Monoclonal Antibody (bsm-52966R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Flow cytometric analysis of Hela cells with MyoD1 (4C4) Monoclonal Antibody (bsm-52966R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).