bsm-52916R
[Primary Antibody]
CD73 Recombinant Antibody
DATASHEET
Host: Rabbit
Target Protein: CD73
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 4907
Swiss Prot: P21589
Source: Human CD73 between 1 to 100 amino acids.
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Store at -20°C for 12 months.
Background:
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
A431 cells lysates probed with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin
Paraformaldehyde-fixed and paraffin-embedded Zebrafish tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin
IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in NIH-3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed, paraffin embedded Human kidney tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD73 (2C9) Monoclonal Antibody, Unconjugated (bsm-52916R) at 1:200 overnight at 4°C, DAB staining.
IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed, paraffin embedded Human endometrial carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD73 (2C9) Monoclonal Antibody, Unconjugated (bsm-52916R) at 1:200 overnight at 4°C, DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with CD73 monoclonal antibody, unconjugated (bsm-52916R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD73 Monoclonal Antibody, Unconjugated(bsm-52916R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD73 Monoclonal Antibody, Unconjugated(bsm-52916R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD73 Monoclonal Antibody, Unconjugated(bsm-52916R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD73 Monoclonal Antibody, Unconjugated(bsm-52916R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.