bsm-52897R
[Primary Antibody]
UCHL1 (2A1) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: UCHL1
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 7345
Swiss Prot: P09936
Source: N terminal human UCHL1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
A549 cells lysates probed with PGP9.5 (2A1) Monoclonal Antibody (bsm-52897R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with PGP9.5 (2A1) Monoclonal Antibody (bsm-52897R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with PGP9.5 (2A1) Monoclonal Antibody (bsm-52897R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with PGP9.5 (2A1) Monoclonal Antibody (bsm-52897R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse spinal cord tissue incubated with PGP9.5 (2A1) Monoclonal Antibody (bsm-52897R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.