DATASHEET
Host:
Rabbit
Target Protein:
C3
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
718
Swiss Prot:
P01024
Source:
Human C3 between 1200 amino acids to the C-terminus
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3-beta-c: Acts as a chemoattractant for neutrophils in chronic inflammation. Acylation stimulating protein: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed:837664, PubMed:29953, PubMed:959512, PubMed:1432298, PubMed:15833747, PubMed:16333141, PubMed:1961575).
VALIDATION IMAGES
Flow cytometric analysis of HepG2 cells with C3 (5C2) Monoclonal Antibody (bsm-52876R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: HepG2 lysates; Lane 2: Hela lysates; Probed with C3 (5C2) Monoclonal Antibody (bsm-52876R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with C3 (5C2) Monoclonal Antibody (bsm-52876R) at 1:80 in NIH-3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.