bsm-52866R
[Primary Antibody]
alpha 1 Antitrypsin (9C7) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: alpha 1 Antitrypsin
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 5265
Swiss Prot: P01009
Source: Full length protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: human spleen muscle lysates; Probed with alpha 1 Antitrypsin (9C7) Monoclonal Antibody (bsm-52866R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with alpha 1 Antitrypsin (9C7) Monoclonal Antibody (bsm-52866R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with alpha 1 Antitrypsin (9C7) Monoclonal Antibody (bsm-52866R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with alpha 1 Antitrypsin (9C7) Monoclonal Antibody (bsm-52866R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with alpha 1 Antitrypsin (9C7) Monoclonal Antibody (bsm-52866R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Human Plasma probed with AACT Monoclonal Antibody, Unconjugated (bsm-52866R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with alpha 1 Antitrypsin (9C7) Monoclonal Antibody (bsm-52866R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.