bsm-52854R
[Primary Antibody]
PDHA1 (3C9) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: PDHA1
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 5160
Swiss Prot: P08559
Source: C terminal human PDHA1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Flow cytometric analysis of Hela cells with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: 293T lysates; Lane 2: A431 lysates; Line 3: Mouse heart lysates ;Probed with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at 1:100 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at 1:100 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at 1:100 in SH-SY-5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human lung cancer tissue incubated with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse skeletal muscle tissue incubated with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.