VALIDATION IMAGES
Flow cytometric analysis of Hela cells with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: 293T lysates; Lane 2: A431 lysates; Line 3: Mouse heart lysates ;Probed with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at 1:100 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at 1:100 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at 1:100 in SH-SY-5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human lung cancer tissue incubated with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse skeletal muscle tissue incubated with PDHA1 (3C9) Monoclonal Antibody (bsm-52854R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.