DATASHEET
Host:
Rabbit
Target Protein:
Cardiac Troponin I
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
7137
Swiss Prot:
P19429
Source:
Human Cardiac Troponin I between 1 to 150 amino acids
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
VALIDATION IMAGES
Human heart lysates probed with Cardiac Troponin I (4C2) Monoclonal Antibody (bsm-52818R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Cardiac Troponin I (4C2) Monoclonal Antibody (bsm-52818R) at 1:100 in NIH/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human heart tissue incubated with Cardiac Troponin I (4C2) Monoclonal Antibody (bsm-52818R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.