bsm-52811R [Primary Antibody]
LYVE1 Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: LYVE1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 10894

Swiss Prot: Q9Y5Y7

Source: Full length protein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of Huvec cells with LYVE1 (5C1) Monoclonal Antibody (bsm-52811R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: MCF-7 lysates; Probed with LYVE1 (5C1) Monoclonal Antibody (bsm-52811R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with LYVE1 (5C1) Monoclonal Antibody (bsm-52811R) at 1:100 in A431 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with LYVE1 (5C1) Monoclonal Antibody (bsm-52811R) at 1:100 in SW480 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with LYVE1 (5C1) Monoclonal Antibody (bsm-52811R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Blood cell lysates; Lane 3: Mouse Lymph node tissue lysates ; Lane 4: Mouse Large intestine tissue lysates ; Lane 5: Rat Thyroid gland tissue lysates ; Lane 6: Rat Spleen tissue lysates ; Lane 7: Rat Lymph node tissue lysates ; Lane 8: Rat Large intestine tissue lysates ; Lane 9: Human Huvec cell lysates ; Lane 10: Human K562 cell lysates probed with LYVE-1 Polyclonal Antibody, Unconjugated (bsm-52811R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.