bsm-52798R
[Primary Antibody]
MMP13 (4C4) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: MMP13
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 4322
Swiss Prot: P45452
Source: N terminus human MMP13
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Flow cytometric analysis of HeLa cells with MMP13 (4C4) Monoclonal Antibody (bsm-52798R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: MCF-7 cells lysates; Probed with MMP13 (4C4) Monoclonal Antibody (bsm-52798R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with MMP13 (4C4) Monoclonal Antibody (bsm-52798R) at 1:300 in HeLa cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with MMP13 (4C4) Monoclonal Antibody (bsm-52798R) at 1:300 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with MMP13 (4C4) Monoclonal Antibody (bsm-52798R) at 1:300 in SW480 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with MMP13 (4C4) Monoclonal Antibody (bsm-52798R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human lung cancer tissue incubated with MMP13 (4C4) Monoclonal Antibody (bsm-52798R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human breast tissue incubated with MMP13 (4C4) Monoclonal Antibody (bsm-52798R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.