bsm-52792R [Primary Antibody]
67kDa Laminin Receptor (8C5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: 67kDa Laminin Receptor

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3921

Swiss Prot: P08865

Source: C terminus human 67kDa Laminin Receptor

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of MCF7 cells with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: K562 lysates; Lane 2: HepG2 lysates; Line 3: A431 lysates; Probed with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human stomach carcinoma tissue incubated with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.