Host: Rabbit
Target Protein: Mannose Receptor(CD206)
Immunogen Range: 1400-1456/1456
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 4360
Swiss Prot: P22897
Source: Full length protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: Lot dependent
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
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Lane 1: Human lung lysates; Lane 2: HepG2 lysates; Line 3: 293T lysates; Probed with Mannose Receptor(CD206) (1C4) Monoclonal Antibody (bsm-52791R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Mannose Receptor(CD206) (1C4) Monoclonal Antibody (bsm-52791R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Mannose Receptor(CD206) (1C4) Monoclonal Antibody (bsm-52791R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Lane 1: Human HepG2 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human 293T cell lysates; Lane 4: Human MCF-7 cell lysates ; Lane 5: Human Molt-4 cell lysates probed with Mannose Receptor(CD206) (1C4) Monoclonal Antibody, Unconjugated (bsm-52791R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
The Ramos(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-MRC1 antibody (bsm-52791R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-60295G-FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with MRC1 monoclonal antibody, unconjugated (bsm-52791R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.