Host: Rabbit
Target Protein: FUS/TLS
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 2521
Swiss Prot: P35637
Source: Recombinant protein within Human FUS aa 350 to the C-terminus
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Flow cytometric analysis of HeLa cells with FUS/TLS (8C1) Monoclonal Antibody (bsm-52774R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
K562 cell lysate probed with FUS/TLS (8C1) Monoclonal Antibody (bsm-52774R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with FUS/TLS (8C1) Monoclonal Antibody (bsm-52774R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with FUS/TLS (8C1) Monoclonal Antibody (bsm-52774R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with FUS/TLS (8C1) Monoclonal Antibody (bsm-52774R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Jurkat cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CCR7 Monoclonal Antibody(bsm-52774R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).