Host: Rabbit
Target Protein: COX4
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 1327
Swiss Prot: P13073
Source: Human COX4 between 1 to 100 amino acids
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Xuena Zhang. et al. Luteoloside Prevents Sevoflurane-induced Cognitive Dysfunction in Aged Rats via Maintaining Mitochondrial Function and Dynamics in Hippocampal Neurons. NEUROSCIENCE. 2023 FebRead more>>
Lane 1: Mouse heart; Lane 2: Rat heart; Probed with COX4 (1C4) Monoclonal Antibody (bsm-52750R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with COX4 (1C4) Monoclonal Antibody (bsm-52750R) at 1:100 in NIH/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with COX4 (1C4) Monoclonal Antibody (bsm-52750R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with COX4 (1C4) Monoclonal Antibody (bsm-52750R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with COX4 (1C4) Monoclonal Antibody (bsm-52750R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with COX4 (1C4) Monoclonal Antibody (bsm-52750R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with COX4 (1C4) Monoclonal Antibody, Unconjugated (bsm-52750R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with COX4 (1C4) Monoclonal Antibody, Unconjugated (bsm-52750R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Human HepG2 cell lysates; Lane 2: Human Hela cell lysates probed with COX4 Monoclonal Antibody, Unconjugated (bsm-52750R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.