bsm-52740R
[Primary Antibody]
Caspase-10 (3C5) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Caspase-10
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 843
Swiss Prot: Q92851
Source: Human Caspase-10 aa 1-250
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. Isoform C is proteolytically inactive.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Flow cytometric analysis of K562 cells with Caspase-10 (3C5) Monoclonal Antibody (bsm-52740R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
K562 cell lysate probed with Caspase-10 (3C5) Monoclonal Antibody (bsm-52740R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Caspase-10 (3C5) Monoclonal Antibody (bsm-52740R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.