bsm-52710R [Primary Antibody]
14-3-3 alpha+beta (3C5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: 14-3-3 alpha+beta

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7529

Swiss Prot: P31946

Source: Synthetic peptide corresponding to C terminal human 14-3-3 alpha + beta

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of HeLa cells with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: Hela Cells; Lane 2: 293T Cells; Lane 3: HepG2 Cells; Probed with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:300 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:300 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:300 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human breast tissue incubated with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse skin tissue incubated with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.