bsm-52703R
[Primary Antibody]
Collagen VI (9C1) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Collagen VI
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 1291
Swiss Prot: P12109
Source: N terminus human Collagen VI
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Collagen VI acts as a cell-binding protein.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: Mouse heart; Lane 2: Human heart; Lane 3: Human kidney; Probed with Collagen VI (9C1) Monoclonal Antibody (bsm-52703R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Collagen VI (9C1) Monoclonal Antibody (bsm-52703R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Collagen VI (9C1) Monoclonal Antibody (bsm-52703R) at 1:300 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Collagen VI (9C1) Monoclonal Antibody (bsm-52703R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with Collagen VI (9C1) Monoclonal Antibody (bsm-52703R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with Collagen VI (9C1) Monoclonal Antibody (bsm-52703R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with Collagen VI (9C1) Monoclonal Antibody (bsm-52703R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.