bsm-52689R
[Primary Antibody]
Tyrosine Hydroxylase (3T1) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Tyrosine Hydroxylase
Clonality: Monoclonal
Isotype: IgG
Swiss Prot: P07101
Source: Human Tyrosine Hydroxylase between 500 to the C-terminus
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Neuronal Marker Tyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Flow cytometric analysis of sh-sy5y cells with Tyrosine Hydroxylase (3T1) Monoclonal Antibody (bsm-52689R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: PC-12 Cells; Lane 2: Mouse brain; Probed with Tyrosine Hydroxylase (3T1) Monoclonal Antibody (bsm-52689R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Tyrosine Hydroxylase (3T1) Monoclonal Antibody (bsm-52689R) at 1:300 in N2A cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Tyrosine Hydroxylase (3T1) Monoclonal Antibody (bsm-52689R) at 1:300 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with Tyrosine Hydroxylase (3T1) Monoclonal Antibody (bsm-52689R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with Tyrosine Hydroxylase (3T1) Monoclonal Antibody (bsm-52689R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.