bsm-52684R
[Primary Antibody]
MEK2 (2C1) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: MEK2
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 5605
Swiss Prot: P36507
Source: Human MEK2 aa 1-100
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Flow cytometric analysis of HeLa cells with MEK2 (2C1) Monoclonal Antibody (bsm-52684R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Jurkat Cells; Lane 2: Hela Cells; Lane 3: 293T Cells; Probed with MEK2 (2C1) Monoclonal Antibody (bsm-52684R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with MEK2 (2C1) Monoclonal Antibody (bsm-52684R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with MEK2 (2C1) Monoclonal Antibody (bsm-52684R) at 1:300 in NIH/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with MEK2 (2C1) Monoclonal Antibody (bsm-52684R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.