DATASHEET
Host:
Rabbit
Target Protein:
PAX6
Immunogen Range:
250-350/422
Clonality:
Monoclonal
Isotype:
IgG
Swiss Prot:
P26367
Source:
Human PAX6 between 250 to 350 amino acids
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.
VALIDATION IMAGES
Flow cytometric analysis of sh-sy5y cells with PAX6 (8T3) Monoclonal Antibody (bsm-52682R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Mouse eye lysate probed with PAX6 (8T3) Monoclonal Antibody (bsm-52682R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Rat eyes tissue incubated with PAX6 (8T3) Monoclonal Antibody (bsm-52682R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse eyes tissue incubated with PAX6 (8T3) Monoclonal Antibody (bsm-52682R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.