bsm-52680R [Primary Antibody]
AMPK beta 1 (6C1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: AMPK beta 1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5564

Swiss Prot: Q9Y478

Source: Human AMPK beta 1 between 150-250 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of A431 cells with AMPK beta 1 (6C1) Monoclonal Antibody (bsm-52680R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


A431 cell lysate probed with AMPK beta 1 (6C1) Monoclonal Antibody (bsm-52680R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.