bsm-52641R [Primary Antibody]
E2F2 (4A6) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: E2F2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 1870

Swiss Prot: Q14209

Source: Recombinant Human E2F2 1 to 110 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of K562 cells with E2F2 (4A6) Monoclonal Antibody (bsm-52641R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: K562 Cells; Lane 2: 293T Cells; Lane 3: HepG2 Cells; Probed with E2F2 (4A6) Monoclonal Antibody (bsm-52641R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.