bsm-52637R
[Primary Antibody]
Cyclin H (5G11) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Cyclin H
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 902
Swiss Prot: P51946
Source: N terminus human Cyclin H
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Flow cytometric analysis of Hela cells with Cyclin H (5G11) Monoclonal Antibody (bsm-52637R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
K562 cells Probed with Cyclin H (5G11) Monoclonal Antibody (bsm-52637R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Cyclin H (5G11) Monoclonal Antibody (bsm-52637R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Cyclin H (5G11) Monoclonal Antibody (bsm-52637R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Cyclin H (5G11) Monoclonal Antibody (bsm-52637R) at 1:300 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with Cyclin H (5G11) Monoclonal Antibody (bsm-52637R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Cyclin H (5G11) Monoclonal Antibody (bsm-52637R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.