VALIDATION IMAGES
Flow cytometric analysis of Hela cells with Cyclin H (5G11) Monoclonal Antibody (bsm-52637R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
K562 cells Probed with Cyclin H (5G11) Monoclonal Antibody (bsm-52637R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Cyclin H (5G11) Monoclonal Antibody (bsm-52637R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Cyclin H (5G11) Monoclonal Antibody (bsm-52637R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Cyclin H (5G11) Monoclonal Antibody (bsm-52637R) at 1:300 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with Cyclin H (5G11) Monoclonal Antibody (bsm-52637R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Cyclin H (5G11) Monoclonal Antibody (bsm-52637R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.