DATASHEET
Host:
Rabbit
Target Protein:
NOX2/gp91phox
Clonality:
Recombinant
Isotype:
IgG
Entrez Gene:
1536
Swiss Prot:
P04839
Source:
Human NOX2/gp91phox aa 150-250
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage:
Store at -20°C for 12 months.
Background:
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
VALIDATION IMAGES
Lane 1: Human A549 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human MCF-7 cell lysates probed with NOX2/gp91phox Monoclonal Antibody, Unconjugated (bsm-52603R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with NOX2 monoclonal antibody, unconjugated (bsm-52603R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.