bsm-52569R [Primary Antibody]
COX1/Cyclooxygenase 1 (4A8) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: COX1/Cyclooxygenase 1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5742

Swiss Prot: P23219

Source: Human COX1/ Cyclooxygenase 1 between 400 to 600 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of HeLa cells with COX1/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: C2C12 Cells; Lane 2: A431 Cells; Probed with COX1/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with COX1/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with COX1/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:300 in N2A cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with COX1/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:300 in C2C12 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with COX1/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


IF(ICC) staining with COX1/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:300 in L6 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Mouse stomach tissue incubated with COX1/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse skin tissue incubated with COX1/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with COX1/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.